Classifications of carbohydrate are monosaccharides, disaccharides, oligosaccharides, and polysaccharides. The students silently read the laboratory procedure from the as an introduction to the laboratory activity. Be specific, but do not re write the lab manual. Record the final color of each test tube in your lab notebook. This test is a test to determine whether or not the carbohydrate contains a free aldehyde or ketone group as indicated above.
Monosaccharides give a rapid positive test. Which macromolecule are the dark granules within the potato cells composed of? Emulsifiers are molecules have both polar and nonpolar parts and thus are capable of dissolving in or interacting with both lipids and water. Data Type of carbohydrate Benedicts Test. If you bond one glucose molecule with a galactose molecule you get lactose, which is commonly found in milk. The iodine molecules slip inside of the amylase coil. Glucose and fructose are monosaccharide.
If you cannot get it thin enough, press down on the cover glass to crush the specimen. The water in test tube No. Maltose Polysaccharides Most of the carbohydrates found in nature occur in the form of high molecular weight polymers called polysaccharides. Most carbohydrates are naturally occurring in plant-based foods, such as grains. Obtain an unknown solution from your laboratory instructor. In the lipids test, petri dishes were used to split the crackers and lemonade power. This is known as the excited state.
Positive test for monosaccharides is the appearance of red precipitate Cu2O within 1-2 minutes. The materials were provided by Mrs. The supportive class culture allows the classroom lab supplies to be well taken care of, as well as extends to the team effort in accomplishing our academic tasks in our daily activities. Add several drops of water while crushing. Any changes to the colour are observed. Add albumin solution to the 2 cm mark to test tubes 2 and 3.
The chair form is the more stable of the two. Use a clean, disposable pipette to transfer several drops of the potato juice in test tube 6 and then fill it to the 1 cm mark with water. Its structure is identical to glycogen, except for a much lower degree of branching about every 20—30 residues. Perform each of the carbohydrate diagnostic tests on the unknown. Sucrose is found in table sugar, and is often formed as a result of photosynthesis sunlight absorbed by chlorophyll reacting with other compounds in plants. The methods that have been learned thus far for identifying bacteria have been applied to this unknown. Once the eight solutions have been prepared on the well plate, students place two drops of the iodine solution in each well.
Lactose which is disaccharides also called reducing sugar as it has the exposed carbonyl groups. Introduction In a lab today, I am to identify an unknown bacterium that is. This means that more than one test has to be performed in order to deduce which compound is present by process of elimination. How are monosaccharides related to di- and polysaccharides? Polysaccharides function as storage materials, structural components, or protective substances. However, the excited configuration is unstable, and the electrons must return back to their positions in the lower energy level. The one on the left contains oil, water, and a detergent. Halides,such as Cl-, Br-, and I- will be used to make these determinations.
Ketoses react within 1 minute of heating while aldoses will require several minutes. The contents of test tubes can be disposed down the drain. The spatial relationships of the atoms of the furanose and pyranose ring structures are more correctly described by the two conformations identified as the chair form and the boat form. All monosaccharides are reducing sugars; they all have a free reactive carbonyl group. Section of a glycogen polymer depicting glucose monomers as colored balls. Alkaline earth metal, Calcium, Chemical element 662 Words 6 Pages In the lab they want determine the concentration of a solution. Each Friday I will ask to see your completed prelab before I allow you to enter the lab.
If you spill any reagent or acid, immediately notify your laboratory instructor. In order to complete this anticipatory activity, students read the text and respond in short answer to the accompanying comprehension questions. They are the fats that store energy. Red brick precipitate is formed for positive results. Pentoses produce furfural acid, while hexoses produce hydroxymethyfurfural. The monomers are reducing sugars which gave the positive result on the second reducing sugar test. Fructose, lactose, and glucose are reducing sugars which give positive test.